Small and medium-sized protein purification system

SePure plus medium pressure glass chromatography column

Introduction:

The chromatographic column is mainly based on the effective plant ingredients (polysaccharides, saponins, flavonoids, alkaloids, etc.), chemical intermediates (amino acids, proteins, nucleic acids, antibiotics, vitamins, etc.), active substances and other substances to be separated and the different fillers in the chromatographic column (macroporous resin, ion exchange resin, gel, silica gel, fibers, etc.):

1、 At different affinities,

2、 Under different concentrations of acidic and alkaline solutions,

3、 Under different polar solutions,

4、 Under different pressures and flow rates

Hierarchical separation of different categories or components to obtain effective components or ingredients for the plan

Column structure: high borosilicate glass column, upper flange, conversion joint (axial expansion), sealing ring, lower flange, inlet and outlet (PTFE)

Column material: high borosilicate glass

Column pressure: 3-6 bar

Corrosion resistance: Acid, alkali, and organic solvents (acetone, methane, ammonia, dichloromethane, etc. Trichloromethane cannot run for a long time)

Axial expansion: With axial expansion function, adjust the height of the filling material, compact the filling material, and increase the pressure inside the column to increase the separation effect

Operating temperature: 0 ℃ -60 ℃

Sieve mesh number: 50 mesh -500 mesh, determined according to the thickness of customer filler. It can completely block the general dextran gel, agarose gel, as well as 400 mesh ion exchange resin and macroporous adsorption resin.

Column cleaning: Soap water and detergent are suitable cleaning agents for chromatography columns. If the chromatography column is contaminated with proteins, detergent can be used for cleaning

Column disinfection: Below 120 ℃, high-pressure disinfection for 20 minutes.